DNA from George Gipp's recently exhumed body shows he's not the father of a child born shortly after his death.
Relatives say the discovery quells longstanding rumors.
Gipp's remains were taken from a cemetery last month for testing near the Upper Peninsula village of Laurium.
Gipp died in 1920 from pneumonia and a strep infection during his senior year at Notre Dame. He's known for the deathbed exhortation "win one for the Gipper."
The DNA testing of the bone was conducted at a laboratory in Dallas, Texas. Results this week showed no link between Gipp and Eva Bright, a South Bend, Indiana woman Gipp had dated for about a year before his death.